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Can pesticides fail PCR tests to detect black mold and microbes?

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Labs and regulators want testing cannabis More and more pollutants to ensure a high level in the market. However, these tests, while critically important, are never perfect. There is an uphill battle while (trying) to prevent a wave of aspergillosis without killing the high-quality products. Overcoming this problem with more detailed laboratory results means that it is certain Insecticides PCR tests for black mold may fail due to hidden GMOs. But this depends on each specific formulation.

Some authorized fungicides use a benign fungicide as the active ingredient. these the benefits Ward off invasive species of mold and mildew, and preserve yield and quality. However, some ingredients may carry contaminants or recombinants, which may force the product to treat under strict guidelines; It’s a sacrifice regardless.

Is PCR better than total colony versus transgenic IP?

Total Colony Forming Units (CFU) is a test to quantify the amount of bacterial contamination. It is not without limitations, because we need to develop specific tests to identify the few species that can colonize. Samples are grown on different growth media, and a human or computer calculates the total colony of cells. Different mold colonies can be better identified by polymerase chain reaction assay.

At the very least, one might assume that a molecular test is higher than the number of CFU. PCR tests pick up a piece of genetic material, and repeat that piece until enough samples have been collected and identified. However, molecular tests suffer from their own limitations. PCR, for example, cannot recognize live and dead cells without prior separation, so benign fungi will still give a positive result.

PCR (polymerase chain reaction) test. Image by Analogicus with permission from Pixabay.

Modified genetics of black mold, a goal and a problem

Trichoderma is a natural mold important for agricultural soil health. But the strain, KRL-AG2 by Bioworks, was once genetically modified using the DNA of a black mold, Aspergillus niger. Here, the forms of limitations; That is, the PCR test may detect the genetics of mold or bacteria that are intentionally introduced into an authorized component. So that particular gene can lead to a false positive result. To avoid all this, KRL-AG2 has a colony-forming assay based on specific growth media and morphology that will not be affected by recombinant genetics.

Subsequently, Bioworks relinquished the intellectual property for the genetically modified Trichoderma in 2009. However, in 2002, it listed the harmful strain of black mold as a potential contaminant. The pesticide product has since updated its formula according to an email response from its Director of Technical Services.

The current Trichoderma is not genetically modified and does not include genes intentionally introduced from Flavalos or Aspergillus. As far as we know, our formulation doesn’t do that [and should not] contain any Flavalos or Aspergillus Niger.

Irfan Vafaei, Ph.D. – Bioworks

Pesticides can be contaminated with microbes but may also contain genetically modified IP and may fail PCR tests, accordingly.

Checking each other’s work for cleaner pesticides and probiotics

Dr. Favey mentioned new measurable information that suggests it would be useful to explore otherwise. Moreover, PCR tests may still give a false-positive result or fail—if another company formulates their pesticides using a similar recombinant technology. But pesticides, it seems, are only part of the battle to ensure that polluting benefits and genetics do not enter the farm.

Kyle Boyar, Research Associate at University of California San Diego Skaggs School From pharmacology has extensive experience in the field of cannabis testing, recently published a the classroom About the nuances of microbial tests in the book “Recent Advances in Cannabis Science”. Boyar’s concerns center around mold and mildew in the hemp field and their surprising sources.

Anecdotal reports indicate that probiotic products used by some farmers have contributed to some Aspergillus contamination. A lesson for farmers, considering that the contamination was only detected by third-party tests. Although authorized pesticides are controlled by stricter regulations than probiotics and nutrients, mistakes can still be made. Peer review process, where Licensed Producers Tested by third-party pesticides themselves, it appears to be ideal for plant viability and consumer safety.

Tell us in the comments what you think of the use of pesticides in cannabis industry. Have you ever planted a lawn and been plagued by a case of powdery mildew?

Resources

  1. US20090104165
  2. Tutt, J.; Magan, N.; brain, p.; Xu, X. Critical evaluation of two commercial biocontrol agents in terms of their efficacy against B. cinerea in in vitro and in vivo conditions with respect to different abiotic agents. Agrology 2021, 11, 1868.
  3. Punja, ZK, Collyer, D., Scott, C., Lung, S., Holmes, J., & Sutton, D. (2019). Pathogens and molds that affect production and quality cannabis NS. Frontiers in BotanyAnd 10, 1120.
  4. PMRA. A biological fungicide from RootShield, Trichoderma harzianum Rifai strain KRL-AG2. 2009. Gov Can.

footnote(s)

https://doi.org/10.3390/agronomy11091868
https://doi.org/10.3389/fpls.2019.01120



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