PubMed: Urinary excretion profile of cannabis analyzes after acute administration of oral and vaporized cannabis in a small number of cannabis users
J Anal Toxicol. 2022 June 29: bkac042. doi: 10.1093/jat/bkac042. online before printing.
Traditionally, smoking was the dominant method of administering cannabis, but alternative methods of use are becoming more prevalent. In addition, research examining urinary cannabinoid excretion profiles has mainly focused on 11-nor-9-carboxy-∆9-tetrahydrocannabinol (Δ9-THC-COOH), the metabolite of Δ9-tetrahydrocannabinol (Δ9-THC), as the primary analysis. The aim of the present study was to characterize the urinary excretion profile of Δ9-THC-COOH, Δ9-THC, Δ8-tetrahydrocannabinol (Δ8-THC), 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-Δ9-THC), Δ9-tetrahydrocannabinol hydrocannabivirine (THCV-COOH), 11-nor-9-tetrahydrocannabivarin-9-carboxylic acid (THCV-COOH), cannabidiol (CBD), cannabinol (CBN), and 811-dihydroxyethyrocannabinol-Δ9-THC after oral and vaporized cannabis administration . Participants (N = 21, 11 men/10 women) who were rarely cannabis users ate cannabis-containing muffins (0, 10, and 25 mg 9-THC) and inhaled cannabis vapors (0, 5, and 20 mg 9-THC) across six Double-blind outpatient sessions. Urinary concentrations of Δ9-THC were measured at baseline and 8 hours after cannabis ingestion. Sensitivity, specificity, and agreement between the three immunoassays (IA) for Δ9-THC-COOH (20, 50, and 100 ng/ml cut-offs) and liquid chromatography-tandem mass spectrometry (LC-MS-MS) (confirmation cut-off) concentrations of 15 were evaluated. ng/ml). Urinary concentrations of Δ9-THC-COOH, Δ9-THC, 11-OH-Δ9-THC, THCV, CBN, and 8,11-diOH-Δ9-THC all peaked at 5-6 hours and 4 hours after oral and vaporized cannabis administration. , Straight. At each active dose, the mean maximum concentrations (Cmax) of the analysis detected were quantitatively higher after oral administration of cannabis compared to vaporization. Using federal recommended workplace drug testing standards (screening by immunoassay with cut-off ≥ 50 ng/ml and confirmation via LC-MS-MS at cut-off ≥ 15 ng/ml), urine samples were tested positive for Δ9-THC-COOH in 97.6% of oral sessions and 59.5% of vaporized sessions with active Δ9-THC doses. These data suggest that while Δ9-THC-COOH may serve as the most consistent confirmatory analysis under current drug testing guidelines, future work examining 11-OH-Δ9-THC against similar criteria could yield an alternative analysis that may be useful. in discrimination. between legal and illegal cannabis products.
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